Data related to 141 sessions of 10 semi-professional basketball players were analyzed during the competitive period of the 2018-2019 season using a multivariable model to determine possible associations between internal and external load variables and fatigue. Age, height, weight, sessional rate of perceived exertion (sRPE), summated-heart-rate-zones, heart rate variability, total accelerations and decelerations were the covariates, and post-session countermovement jump loss (10% or higher) the response variable. Based on the results observed, a rise in sRPE and accelerations and decelerations could be associated with increased lower-body neuromuscular fatigue. Observing neuromuscular fatigue was 1,008 times higher with each additional sRPE arbitrary unit (AU). Each additional high-intensity effort also increased the probability of significant levels of neuromuscular fatigue by 1,005 times. Fatigue arising from demanding sporting activities is acknowledged as a relevant inciting event leading to injuries. Thus, the methodology used in this study can be used then to monitor neuromuscular fatigue onset, also enhancing proper individual adaptations to training.

• Several internal and external load variables show associations with an increase in local neuromuscular fatigue detected through the countermovement jump test.

• Our main findings show promising results detecting the lower-body neuromuscular fatigue originated by specific training through and ecological, practical, and low time-consuming methodology. The proposed method can outline the players’ physiological state and inform decisions regarding the application of different training loads within a complex setting and multi-factorial process.